Kamal-Eldin, Afaf
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
Androstenone (5 alpha-androst-16-en-3-one) is a major component in boar taint. Here, a liquid chromatography-tandem mass spectrometry method was developed to analyse androstenone in porcine plasma to facilitate studies of its transport from pig testes to adipose tissues. The method used androstanone (5 alpha-andro-stan-3-one) as internal standard, Oasis (R) MCX solid-phase extraction, C18 reversed-phase column, and API 5000 Triple Quadrupole mass spectrometry with positive electrospray ionisation interface operating in the multiple reaction monitoring mode. Incubation of the plasma samples with beta-glucuronidase/arylsulfatase revealed an increase in androstenone yield indicating the presence of a conjugated form of its 3-enol isomer. With this method the limit of detection (LOD) was 1.0 ng and the limit of quantification (LOQ) was 3.3 ng per mL of plasma. In conclusion, the presented method is sensitive and reproducible and thus suitable for accurate quantification of androstenone levels in a small volume of porcine plasma. (C) 2010 Elsevier Ltd. All rights reserved.
Keywords
Androstenone; Porcine plasma; Solid-phase extraction; LC-MS/MS
Published in
Food Chemistry
2010, volume: 122, number: 4, pages: 1278-1282
Publisher: ELSEVIER SCI LTD
SLU Authors
Lundström, Kerstin
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/j.foodchem.2010.03.083
Permanent link to this page (URI)
https://res.slu.se/id/publ/61173