Abstract

In a flash diffraction experiment, a short and extremely intense x-ray pulse illuminates the sample to obtain a diffraction pattern before the onset of significant radiation damage. The over-sampled diffraction pattern permits phase retrieval by iterative phasing methods. Flash diffractive imaging was first demonstrated on an inorganic test object (Chapman et al 2006 Nat. Phys. 2 839-43). We report here experiments on biological systems where individual cells were imaged, using single, 10-15 fs soft x-ray pulses at 13.5 nm wavelength from the FLASH free-electron laser in Hamburg. Simulations show that the pulse heated the sample to about 160 000 K but not before an interpretable diffraction pattern could be obtained. The reconstructed projection images return the structures of the intact cells. The simulations suggest that the average displacement of ions and atoms in the hottest surface layers remained below 3 angstrom during the pulse.

Published in

Journal of Physics B: Atomic, Molecular and Optical Physics
2010, Volume: 43, number: 19, article number: 194015
Publisher: IOP PUBLISHING LTD

SLU Authors

• Andersson, Inger

  • Department of Molecular Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Atom and Molecular Physics and Optics


Publication identifier

DOI: https://doi.org/10.1088/0953-4075/43/19/194015

Permanent link to this page (URI)

https://res.slu.se/id/publ/61210