Bertoft, Eric
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
Cassava and rice amylopectins and their phi,beta-limit dextrins were debranched and the unit chain compositions were analyzed by gel permeation chromatography and high performance anion-exchange chromatography The content of amylose and super-long amylopectin chains was 15 5-15.6% and 0.8-1.2% in cassava and 15.1-18.7% and 3 4-8 6% in rice starches, respectively. The internal unit chain profiles of the amylopectin samples were obtained from the phi,beta-limit dextrins Shott B-chains were subdivided into a major group (BS(major)) with a degree of polymerization (DP) of 8-25 and a minor group of "fingerprint" B-chains (B(fp)) with DP 3-7. Cassava and rice amylopectins possessed different amounts and patterns of B(fp)-chains The rice samples contained higher amounts of B(fp)-chains, which suggested larger size of its. clusters when compared with cassava. The content of the shortest "fingerprint" A-chains (A(fp)) was also variable, especially between the rice cultivars The position of the internal chains in the original amylopectin chain profile was traced The reconstructed B-chain profiles fitted well with the original profiles. This implied that most of the B-chains carried external chain segments of similar lengths, which corresponded to the experimentally measured average length.
Keywords
Cassava; Rice; Amylopectin, phi,beta-Limit dextrin; Internal structure
Published in
Starch/Stärke
2009, volume: 61, number: 10, pages: 557-569
Publisher: WILEY-V C H VERLAG GMBH
SLU Authors
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1002/star.200900154
Permanent link to this page (URI)
https://res.slu.se/id/publ/61214