Persson, Mattias
- Department of Plant Biology, Swedish University of Agricultural Sciences
Research article2009Peer reviewed
Layers of defense responses to Leptosphaeria maculans below the RLM1- and camalexin-dependent resistances
Persson, Mattias; Staal, Jens; Oide, Shinichi; Dixelius, Christina
Abstract
Plants have evolved different defense components to counteract pathogen attacks. The resistance locus resistance to Leptosphaeria maculans 1 (RLM1) is a key factor for Arabidopsis thaliana resistance to L. maculans. The present work aimed to reveal downstream defense responses regulated by RLM1.Quantitative assessment of fungal colonization in the host was carried out using quantitative polymerase chain reaction (qPCR) and GUS expression analyses, to further characterize RLM1 resistance and the role of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) in disease development. Additional assessments of A. thaliana mutants were performed to expand our understanding of this pathosystem.Resistance responses such as lignification and the formation of vascular plugs were found to occur in an RLM1-dependent manner, in contrast to the RLM1-independent increase in reactive oxygen species at the stomata and hydathodes. Analyses of mutants defective in hormone signaling in the camalexin-free rlm1(Ler)pad3 background revealed a significant influence of JA and ET on symptom development and pathogen colonization.The overall results indicate that the defense responses of primary importance induced by RLM1 are all associated with physical barriers, and that responses of secondary importance involve complex cross-talk among SA, JA and ET. Our observations further suggest that ET positively affects fungal colonization.
Keywords
Arabidopsis thaliana; camalexin; defense responses; hormones; Leptosphaeria maculans; lignin; Phoma lingam; quantitative real-time PCR
Published in
New Phytologist
2009, volume: 182, number: 2, pages: 470-482
Publisher: WILEY-BLACKWELL PUBLISHING, INC
SLU Authors
Oide, Shinichi
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
Dixelius, Christina
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1111/j.1469-8137.2009.02763.x
Permanent link to this page (URI)
https://res.slu.se/id/publ/61659