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Research article - Peer-reviewed, 2014

Quantitative evaluation of synbiotic strategies to improve persistence and metabolic activity of Lactobacillus reuteri DSM 17938 in the human gastrointestinal tract

Rattanaprasert, Monchaya; Roos, Stefan; Hutkins, Robert W.; Walter, Jens

Abstract

The efficacy of three synbiotic approaches to improve persistence and metabolic activity of Lactobacillus reuteri DSM 17938 in the human gut was determined in a single-blind, randomized, crossover, placebo-controlled trial. Synbiotics consisting of 5 x 10(8) bacterial cells and 2 g of either GOS, rhamnose or the combination of both were given to 15 healthy adults for 7 days, followed by 10 days during which only the carbohydrates were administered. Faecal samples were collected and quantitative real-time PCR was used to determine cell numbers and rRNA copy number. Although L. reuteri DSM 17938 was detectable in faecal samples after consumption, the addition of GOS, rhamnose, and the mixture of the both did not increase faecal populations, nor was persistence enhanced after consumption of the probiotic had ended. However, based on rRNA per cell ratios, the combination of GOS and rhamnose significantly stimulated metabolic activity of the L. reuteri strain. (C) 2014 Elsevier Ltd. All rights reserved.

Keywords

Lactobacillus reuteri; Galactooligosaccharide; Rhamnose; 1,2-Propanediol; Synbiotic; Probiotic

Published in

Journal of Functional Foods
2014, Volume: 10, pages: 85-94
Publisher: ELSEVIER SCIENCE BV

    UKÄ Subject classification

    Microbiology
    Food Science

    Publication identifier

    DOI: https://doi.org/10.1016/j.jff.2014.05.017

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/62047