Abstract

The development and activity of microbiota in artificial sediment may have strong implications for the fate of test compounds and the outcome of toxicity tests. In this study, we compare a number of microbial variables in the artificial sediment commonly used in toxicity testing with that of natural sediments. Bacterial abundance of artificial sediment ranged 5.7 to 7.1 X 10(8) cells/g wet weight, which is about two orders of magnitude lower than values commonly reported for natural sediments. Similarly. alternative estimates of microbial biomass (sum of phospholipid fatty acid, ergosterol, adenosine triphosphate) were several times lower for artificial sediment compared with natural sediment. Bacterial activity (H-3-thymidine incorporation) ranged 4.0 to 7.4 pmol g(-1) h(-1) (or 0.062-0.113 mu g C g(-1) h(-1)) in artificial sediments, which is low compared with values commonly reported for freshwater sediments. Community respiration in artificial sediment was 34 to 93 mu g CO2 g(-1) d(-1). Bacterial community composition assessed by terminal restriction fragment length polymorphism of polymerase chain reaction amplified 16S rRNA genes demonstrated that natural bacterial communities appear to be more diverse than their counterparts in artificial sediment. The average similarity of the microbial communities obtained by this method was less than 40%, and different operational taxonomic units appeared to dominate the artificial and natural sediment, respectively. These results and supporting data from previous studies in natural sediments suggest that the artificial sediment has a poorly developed microbial component that differs substantially from that in natural sediments

Published in

Environmental Toxicology and Chemistry
2005, Volume: 24, number: 11, pages: 2725-2733
Publisher: SETAC

SLU Authors

• Goedkoop, Willem

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science
Fish and Aquacultural Science
Environmental Sciences related to Agriculture and Land-use


Publication identifier

DOI: https://doi.org/10.1897/04-523R.1

Permanent link to this page (URI)

https://res.slu.se/id/publ/6421