Abstract

Plants have lysophosphatidylcholine transacylase (LPCT) and acyl-CoA:glycerophosphocholine acyltransferase (GPCAT) activities. The combined action of LPCT and GPCAT provides a novel route of PC re-synthesis after its deacylation.Phosphatidylcholine (PC) is the major lipid in eukaryotic membranes and has a central role in overall plant lipid metabolism. It is also the site of production of polyunsaturated fatty acids in plants. The recently discovered acyl-CoA:glycerophosphocholine acyltransferase (GPCAT) activity in yeast provides a novel route of re-synthesising PC via lysophosphatidylcholine (LPC) after its deacylation. This route does not require the degradation of the glycerophosphocholine (GPC) into free choline, the activation of choline to CDP-choline, nor the utilization of CDP-choline by the CDP-choline:diacylglycerol cholinephosphotransferase. We show here that GPCAT activities also are present in membrane preparations from developing oil seeds of safflower and other species as well as in membrane preparations of roots and leaves of Arabidopsis, indicating that GPCAT activity plays a ubiquitous role in plant lipid metabolism. The last step in formation of GPC, the substrate for GPCAT, is the deacylation of LPC. Microsomal membranes of developing safflower seeds utilized LPC in LPC:LPC transacylation reactions (LPCT activities) creating PC and GPC. The results demonstrate that safflower membranes have LPCT and GPCAT activities that represent novel reactions for PC acyl editing. The physiological relevance of these reactions probably has to await identification of the enzymes catalysing these reactions.

Keywords

Lipid biochemistry; Phosphatidylcholine metabolism; Glycerophosphocholine:acyl-CoA acyltransferase; Lysophosphatidylcholine transferase

Published in

Planta
2015, Volume: 241, number: 2, pages: 347-358
Publisher: SPRINGER

SLU Authors

• Lager, Ida

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Glab, Bartosz

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    
  • University of Gdansk

• Eriksson, Lovisa

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Stymne, Sten

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1007/s00425-014-2184-1

Permanent link to this page (URI)

https://res.slu.se/id/publ/65143