Exogenous sodium sulfide improves morphological and physiological responses of a hybrid Populus species to nitrogen dioxide

Hu, Yanbo; Bellaloui, Nacer; Sun, Guangyu; Tigabu, Mulualem; Wang, Jinghong

doi:10.1016/j.jplph.2013.10.018

Research article2014Peer reviewed

Exogenous sodium sulfide improves morphological and physiological responses of a hybrid Populus species to nitrogen dioxide

Hu, Yanbo; Bellaloui, Nacer; Sun, Guangyu; Tigabu, Mulualem; Wang, Jinghong

Abstract

Gaseous nitrogen dioxide (NO2) can disturb normal plant growth and trigger complex physiological responses. NO2-induced responses are influenced by biotic or abiotic factors. In this study, we investigated the effects of exogenous sodium sulfide (Na2S, 5 mmol L-1) on epidermis and stomata related physicochemical responses of hybrid poplar cuttings (Pouplus alba x P. berolinensis) to gaseous NO2 (4 mu l 1(-1)) for three time periods (0, 14 and 48 h). We also investigated hydrogen sulfide (H2S), nitrate-nitrogen and nitrate reductase activity (NR) in control and Na2S treated plants. Our results showed that NO2 exposure for 48 h led to the decline of NR, maximal PSII quantum yield (F-v/F-m), net photosynthetic rate (P-n), and dark respiration rate (R-d). The maximum rate for the post-illumination carbon dioxide burst (PIB) occurred in 48-h exposed leaves 13-15 s after darkening. Moreover, NO2 exposure resulted in a significant increase in nitrogen percentage (from 0 to 33%) and a decrease in the macro and microelements of leaf surface. Spraying Na2S aqueous solution on the leaf surfaces significantly increased the thicknesses of palisade/spongy tissue and H2S content. Na2S pretreatment alleviated NO2-caused toxic effects as indicated by increased NR and higher values of P-n, F-v/F-m, and actual photochemical efficiency in light (Phi PSII) compared with the control. Na2S pretreatment had no significant impacts on PIB-based photorespiration or elements composition of a leaf surface. (C) 2013 Elsevier GmbH. All rights reserved.

Keywords

Nitrogen dioxide; Nitrate reductase activity; Photosynthesis; Populus trees; Sodium sulfide

Published in

Journal of Plant Physiology
2014, volume: 171, number: 10, pages: 868-875
Publisher: ELSEVIER GMBH, URBAN & FISCHER VERLAG

SLU Authors

Tigabu, Mulualem
- Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences

UKÄ Subject classification

Forest Science

Publication identifier

DOI: https://doi.org/10.1016/j.jplph.2013.10.018

Permanent link to this page (URI)

https://res.slu.se/id/publ/66502