Abstract

Obesity in dogs is increasing and lifestyle-related diseases affect pets as well as pet owners. Urine metabolite profiles have earlier shown to be specific for both dog phenotypes and disease status. The objective of this study was to compare urine metabolite profiles in normal weight and overweight Labrador retrievers in fasting and postprandial samples. A total of 28 healthy intact male dogs aged 1-9 years with body condition score 4-8 (BCS, scale 1-9) were included in the study. Of these dogs 16 were classified as overweight (BCS 6-8) and 12 were classified as normal weight (BCS 4-5). An overnight fasting period of 14-17 hours was followed by collection of free catch morning urine. Thereafter each dog was fed a high energy diet containing half its daily maintenance requirements, based on body weight (BCS 4-5) and calculated ideal body weight (BCS 6-8).Three hours after the meal, a second urine sample was collected. Proton nuclear magnetic resonance spectroscopy combined with multivariate analysis was used for urine evaluation at both time points. Principal component analysis of metabolite profiles showed less variation among postprandial samples than among fasting samples and metabolite profiles changed with age irrespective of sampling time point. Moreover, a difference was seen between normal weight and overweight dogs in postprandial metabolite profiles. Therefore, identification of obesity-related urine metabolites appears possible and promise to provide further knowledge of metabolic alterations in dog obesity. In addition, urine biomarkers merit to be investigated as a potential diagnostic tool for obesity-related metabolic dysfunctions in dogs.

Published in

Journal of Veterinary Internal Medicine
2014, Volume: 28, number: 3, pages: 1090-1090
Publisher: American College of Veterinary Internal Medicine

Conference

American College of Veterinary Internal Medicine (ACVIM) Forum 2014

SLU Authors

• Söder, Josefin

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Hagman, Ragnvi

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Dicksved, Johan

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

• Truelsen Lindåse, Sanna

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Agback, Peter

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Malmlöf, Kjell

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Wernersson, Sara

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Associated SLU-program

Future Animal Health and Welfare (until Jan 2017)


UKÄ Subject classification

Medical Bioscience


Publication identifier

DOI: https://doi.org/10.1111/jvim.12361

Permanent link to this page (URI)

https://res.slu.se/id/publ/66579