Pejler, Gunnar
- Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences
Abstract
We investigated whether human peripheral blood mononuclear cells (PBMC) express prothrombinase following stimulation with bacterial lipopolysaccharide (LPS). LPS-stimulated PBMC devoid of contaminating platelets failed to activate prothrombin directly. Addition of platelets did not result in expression of prothrombinase in the absence of factor X whereas the combination of platelets and factor X resulted in strong prothrombinase activity on LPS-activated cells. The induced prothrombinase was dependent on tissue factor, as the activity was completely inhibited by an anti-tissue factor antibody. Our data suggest that platelet/monocyte cooperation is important in the generation of prothrombinase activity in response to endotoxin.
Keywords
macrophages; prothrombinase; factor X; platelets; tissue factor
Published in
Annals of Hematology
2001, volume: 80, number: 5, pages: 278-283
Publisher: SPRINGER-VERLAG
SLU Authors
Associated SLU-program
Future Animal Health and Welfare (until Jan 2017)
UKÄ Subject classification
Molecular Biology
Biochemistry
Publication identifier
- DOI: https://doi.org/10.1007/s002770100288
Permanent link to this page (URI)
https://res.slu.se/id/publ/66609