Macrophages synthesize Factor X and secrete factor X/Xa-containing prothrombinase activity into the surrounding medium

Abstract

Activation of the coagulation cascade, mediated by various monocyte/macrophage procoagulants, is an important component in the pathology of inflammatory disease. The type of procoagulant expressed may vary between different monocyte/macrophage subtypes and may differ depending on how the cells are treated. In the present study we show that both murine peritoneal macrophages and human adherent synovial cells from rheumatoid arthritis lesions express prothrombinase activity that was inhibited by anti-Factor X antibodies. Northern blot analysis showed that Factor X was transcribed by the murine peritoneal cells and Western blot analysis showed the presence of Factor X antigen. Further experiments showed that the prothrombinase activity was secreted by the cells into the medium in a detergent-sensitive form, suggesting that the prothrombinase is released on small lipid-containing vesicles.

Keywords

macrophage; prothrombinase; Factor X; thrombin

Published in

Thrombosis and Haemostasis
2000, volume: 84, number: 3, pages: 429-435
Publisher: SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN

SLU Authors

• Pejler, Gunnar

  • Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences
    

Associated SLU-program

Future Animal Health and Welfare (until Jan 2017)

UKÄ Subject classification

Biochemistry
Biochemistry and Molecular Biology
Molecular Biology

Publication identifier

• DOI: https://doi.org/10.1055/s-0037-1614040

Permanent link to this page (URI)

https://res.slu.se/id/publ/66610