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Research article - Peer-reviewed, 2014

Loss and retention of resistance genes in five species of the Brassicaceae family

Peele, Hanneke Marjolijn; Guan, Na; Fogelqvist, Johan; Dixelius, Christina

Abstract

Background: Plants have evolved disease resistance (R) genes encoding for nucleotide-binding site (NB) and leucine-rich repeat (LRR) proteins with N-terminals represented by either Toll/Interleukin-1 receptor (TIR) or coiled-coil (CC) domains. Here, a genome-wide study of presence and diversification of CC-NB-LRR and TIR-NB-LRR encoding genes, and shorter domain combinations in 19 Arabidopsis thaliana accessions and Arabidopsis lyrata, Capsella rubella, Brassica rapa and Eutrema salsugineum are presented.Results: Out of 528 R genes analyzed, 12 CC-NB-LRR and 17 TIR-NB-LRR genes were conserved among the 19 A. thaliana genotypes, while only two CC-NB-LRRs, including ZAR1, and three TIR-NB-LRRs were conserved when comparing the five species. The RESISTANCE TO LEPTOSPHAERIA MACULANS 1 (RLM1) locus confers resistance to the Brassica pathogen L. maculans the causal agent of blackleg disease and has undergone conservation and diversification events particularly in B. rapa. On the contrary, the RLM3 locus important in the immune response towards Botrytis cinerea and Alternaria spp. has recently evolved in the Arabidopsis genus.Conclusion: Our genome-wide analysis of the R gene repertoire revealed a large sequence variation in the 23 cruciferous genomes. The data provides further insights into evolutionary processes impacting this important gene family.

Keywords

Arabidopsis thaliana; Brassicaceae; CC/TIR-NB-LRR domains; Genomes; Leptosphaeria maculans; Resistance genes

Published in

BMC Plant Biology
2014, volume: 14, article number: 298
Publisher: BIOMED CENTRAL LTD

Authors' information

Peele, Hanneke Marjolijn
Swedish University of Agricultural Sciences, Department of Plant Biology
Guan, Na
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Biology

Associated SLU-program

SLU Network Plant Protection

UKÄ Subject classification

Bioinformatics and Systems Biology
Plant Biotechnology

Publication Identifiers

DOI: https://doi.org/10.1186/s12870-014-0298-z

URI (permanent link to this page)

https://res.slu.se/id/publ/66819