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Research article2014Peer reviewedOpen access

Comparison of Bovine coronavirus-specific and Bovine respiratory syncytial virus-specific antibodies in serum versus milk samples detected by enzyme-linked immunosorbent assay

Ohlson, Anna; Blanco-Penedo, Isabel; Fall, Nils

Abstract

Bovine coronavirus (BCV; Betacoronavirus 1) and Bovine respiratory syncytial virus (BRSV) are significant causes of enteric and respiratory disease in beef and dairy cattle throughout the world. Indirect enzyme-linked immunosorbent assays are widely used to detect serum antibodies for herd monitoring and prevalence studies. In dairy herds, milk is more readily collected than serum. Hence, in order to investigate the test agreement between serum and milk, both serum and milk samples from 105 cows in 27 dairy herds were analyzed in parallel for presence of immunoglobulin G antibodies to BCV and BRSV. The Bland-Altman analyses of data demonstrated good agreement between serum and milk antibody titers for both viruses. The results indicate milk samples are sufficient for surveillance of antibodies to BCV and BRSV.

Keywords

Antibody correlation; serum; milk; Bovine respiratory syncytial virus; Bovine coronavirus; antibody evaluation; enzyme-linked immunosorbent assay

Published in

Journal of Veterinary Diagnostic Investigation
2014, Volume: 26, number: 1, pages: 113-116
Publisher: SAGE PUBLICATIONS INC

      UKÄ Subject classification

      Clinical Science

      Publication identifier

      DOI: https://doi.org/10.1177/1040638713509377

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/67449