Abstract

A gene encoding a sterol ester synthesizing enzyme was identified in Arabidopsis. The gene belongs to the LCAT/PDAT family and is the only intracellular enzyme so far found which catalyzes an acyl CoA-independent sterol ester formation. It catalyzes a transacylation of acyl groups from phospholipids to sterols. When this gene was knocked out the content of sterol esters were reduced by about 70% in the plant tissue. No difference in the phenotype between the AtPSAT knock out plants and their null segregants was detected at room temperature. However, when the plants were subjected to 2 or 6 degree Celsius, older leaves of the knockout plants started to die off. Signs of this were visible already after 2 – 3 weeks in the 6 degree Celsius and later in the 2 degree treatment. The results presented implicate a role for AtPSAT in regulating both the amount and the quality of the free sterols in the membrane. They also suggest an involvement of AtPSAT in plant cold resistance

Published in

Conference

2nd Swedish Plant Lipid Meeting

SLU Authors

• Carlsson, Anders

  • Department of Crop Science, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science
Food Science


Permanent link to this page (URI)

https://res.slu.se/id/publ/6753