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Research article - Peer-reviewed, 2015

Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease

Balint A, Balka G, Horvath P, Kecskemeti S, Dan A, Farsang A, Szeredi L, Banyai K, Bartha D, Olasz F, Belak S, Zadori Z

Abstract

Here, we report the isolation of a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory problems and high fever. Next-generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, and shows the highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 revealed no evidence of recombination but did show a high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of selective immune pressure in the evolution of PRRSV 9625/2012.

Keywords

PRRSV; Sequencing; Complete genome; Epitope

Published in

Archives of Virology
2015, Volume: 160, number: 2, pages: 417-422

        UKÄ Subject classification

        Medical Bioscience

        Publication identifier

        DOI: https://doi.org/10.1007/s00705-014-2265-2

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/67642