In situ cross-linkable hyaluronan hydrogel enhances chondrogenesis

Abstract

The present work describes the feasibility of a cross-linkable injectable hyaluronan hydrogel for cartilage repair. The hydrogel used is a two-component system based on aldehyde-modified hyaluronan and hydrazide-modified polyvinyl alcohol, which are rapidly cross-linked in situ upon mixing. The in vitro study showed that chondrocytes and mesenchymal cells cultured in the gel form cartilage-like tissue, rich in glycosaminoglycans, collagen type II and aggrecan. In a rabbit animal model the injection of the hydrogel improved the healing of a full-thickness cartilage defect created in the knee as compared to non-treated controls. This rabbit study showed that the regenerated cartilage defects stained more intensely for type II collagen upon treatment with the hydrogel. The hyaluronan-based hydrogel may be used as a delivery vehicle for both growth factors and/or cells for cartilage repair. The in vivo study also indicated that the hydrogel alone has a beneficial effect on cartilage regeneration. Copyright (C) 2011 John Wiley & Sons, Ltd.

Keywords

cartilage; injectable hyaluronan; cross-linked; bone morphogenetic protein-2; rabbit model

Published in

Journal of Tissue Engineering and Regenerative Medicine
2011, volume: 5, number: 8, pages: E188-E196
Publisher: WILEY-BLACKWELL

SLU Authors

• Jensen Waern, Marianne

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Hedenqvist, Patricia

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science
Pharmaceutical and Medical Biotechnology

Publication identifier

• DOI: https://doi.org/10.1002/term.415

Permanent link to this page (URI)

https://res.slu.se/id/publ/68312