Jansson, Ronnie
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Abstract
Enzyme immobilization is an attractive route for achieving catalytically functional surfaces suitable for both continuous and repeated use. Herein, genetic engineering is used to combine the catalytic ability of a xylanase with the self-assembly properties of recombinant spider silk, realizing silk materials with enzymatic activity. Under near-physiological conditions, soluble xylanase-silk fusion proteins assembled into fibers displaying catalytic activity. Also, a xylanase-silk protein variant with the silk part miniaturized to contain only the C-terminal domain of the silk protein formed fibers with catalytic activity. The repertoire of xylanase-silk formats is further extended to include 2D surface coatings and 3D foams, also being catalytically active, showing the versatile range of possible silk materials. The stability of the xylanase-silk materials is explored, demonstrating the possibility of storage, reuse, and cleaning with ethanol. Interestingly, fibers can also be stored dried with substantial residual activity after rehydration. Moreover, a continuous enzymatic reaction using xylanase-silk is demonstrated, making enzymatic batch reactions not the sole possible implementation. The proof-of-concept for recombinantly produced enzyme-silk, herein shown with a xylanase, implies that also other enzymes can be used in similar setups. It is envisioned that the concept of enzyme-silk can find its applicability in, for example, multienzyme reaction systems or biosensors.
Keywords
enzyme immobilization; gene fusion; protein-based materials; recombinant spider silk; xylanase
Published in
Advanced Functional Materials
2015, volume: 25, number: 33, pages: 5343-5352
Publisher: WILEY-V C H VERLAG GMBH
SLU Authors
Sandgren, Mats
- The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
- The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
Hedhammar, My
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Royal Institute of Technology (KTH)
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Global goals (SDG)
SDG12 Responsible consumption and production
UKÄ Subject classification
Materials Chemistry
Biophysics
Publication identifier
- DOI: https://doi.org/10.1002/adfm.201501833
Permanent link to this page (URI)
https://res.slu.se/id/publ/68448