Abstract

Mast cells (MCs) have detrimental functions in the context of numerous pathologies, and regimens aimed at neutralizing MCs or individual MC products can thus be of therapeutic value. One way to target MCs in disease is to selectively induce MC apoptosis, but there is so far no agent available that selectively induces apoptosis in MCs. Mast cells are heavily loaded with secretory granules containing large amounts of fully active proteases bound to serglycin proteoglycan. Damage to the secretory granules will thus lead to the release of serglycin-protease complexes into the cytosol. A potential consequence of this would be that the unleashed granular proteases cause apoptosis by proteolytic activation of proapoptotic compounds located in the cytosol. Indeed, we have recently found that MCs are highly sensitive to apoptosis induced by permeabilization of the secretory granules. In this chapter, we describe the methods used to study MC apoptosis induced by this novel, secretory granule-mediated pathway.

Keywords

Mast cells; Apoptosis; Proteases; Granules; Caspases

Published in

Methods in Molecular Biology
2015, Volume: 1220, number: 1220, pages: 325-337
Title: Mast Cells : methods and protocols
ISBN: 978-1-4939-1567-5
Publisher: Springer New York

SLU Authors

• Rabelo Melo, Fabio

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Wernersson, Sara

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Pejler, Gunnar

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell and Molecular Biology
Medical Bioscience


Publication identifier

DOI: https://doi.org/10.1007/978-1-4939-1568-2_20

Permanent link to this page (URI)

https://res.slu.se/id/publ/68713