Grenville-Briggs Didymus, Laura
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
Abstract
Oomycetes are eukaryotic microorganisms, which are phylogenetically distinct from the true-fungi, which they resemble morphologically. While many oomycetes are pathogenic to plants, Pythium insidiosum is capable of infecting humans and animals. Mitochondrial (mt) genomes are valuable genetic resources for exploring the evolution of eukaryotes. During the course of 454-based nuclear genome sequencing, we identified a complete 54.9 kb mt genome sequence, containing 2 large inverted repeats, from P. insidiosum. It contains 65 different genes (including 2 ribosomal RNA genes, 25 transfer RNA genes and 38 genes encoding NADH dehydrogenases, cytochrome b, cytochrome c oxidases, ATP synthases, and ribosomal proteins). Thirty-nine of the 65 genes have two copies, giving a total of 104 genes. A set of 30 conserved protein-coding genes from the mt genomes of P. insidiosum,11 other oomycetes, and 2 diatoms (outgroup) were used for phylogenetic analyses. The oomycetes can be classified into 2 phylogenetic groups, in relation to their taxonomic lineages: Saprolegnialean and Peronosporalean. P. insidiosum is more closely related to Pythium ultimum than other oomycetes. In conclusion, the complete mt genome of P. insidiosum was successfully sequenced, assembled, and annotated, providing a useful genetic resource for exploring the biology and evolution of P. insidiosum and other oomycetes. (C) 2015 Elsevier B.V. All rights reserved.
Keywords
Pythium insidiosum; Pythiosis; Oomycete; Mitochondrial genome; Phylogenetic analysis
Published in
Gene
2016, volume: 575, number: 1, pages: 34-41
SLU Authors
UKÄ Subject classification
Microbiology in the medical area
Immunology in the medical area
Infectious Medicine
Publication identifier
- DOI: https://doi.org/10.1016/j.gene.2015.08.036
Permanent link to this page (URI)
https://res.slu.se/id/publ/69019