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Research article - Peer-reviewed, 2015

Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda

Rwabiita Mugizi, Denis; Muradrasoli, Shaman; Boqvist, Sofia; Erume, Joseph; Nasinyama, George; Waiswa, Charles; Mboowa, Gerald; Klint, Markus; Magnusson, Ulf


Brucellosis is endemic in livestock and humans in Uganda and its transmission involves amultitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.

Published in

BioMed Research International
2015, number: 720413

Authors' information

Rwabiita Mugizi, Denis
Makerere University
Muradrasoli, Shaman
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Erume, Joseph
Makerere University
Nasinyama, George
Makerere University
Waiswa, Charles
Makerere University
Mboowa, Gerald
Makerere University
Klint, Markus
Uppsala University
Swedish University of Agricultural Sciences, Department of Clinical Sciences

Sustainable Development Goals

SDG3 Good health and wellbeing

UKÄ Subject classification

Medical Bioscience

Publication Identifiers


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