Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda

Abstract

Brucellosis is endemic in livestock and humans in Uganda and its transmission involves amultitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.

Published in

BioMed Research International
2015, number: 720413
Publisher: HINDAWI PUBLISHING CORPORATION

SLU Authors

• Rwabiita Mugizi, Denis

  • Makerere University

• Muradrasoli, Shaman

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Boqvist, Sofia

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Magnusson, Ulf

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

Global goals (SDG)

SDG3 Good health and well-being

UKÄ Subject classification

Medical Bioscience

Publication identifier

• DOI: https://doi.org/10.1155/2015/720413

Permanent link to this page (URI)

https://res.slu.se/id/publ/69405