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Research article - Peer-reviewed, 2015

Isolation of Leptospira interrogans serovar Hardjoprajitno from a calf with clinical leptospirosis in Chile

Salgado, Miguel; Otto, Barbara; Moroni, Manuel; Sandoval, Errol; Reinhardt, German; Boqvist, Sofia; Encina, Carolina; Muñoz-Zanzi, Claudia

Abstract

Background: Although Leptospira isolation has been reported in Chilean cattle, only serological evidence of serovar Hardjo bovis infection has been routinely reported. The present report provides characterization of the pathological presentation and etiology of a clinical case of leptospirosis in a calf from the Los Rios Region in Chile.Case presentation: In a dairy herd in southern Chile, 11 of 130 calves died after presenting signs such as depression and red-tinged urine. One of these calves, a female of eight months, was necropsied, and all the pathological findings were consistent with Leptospira infection. A urine sample was submitted to conventional bacteriological analysis together with highly specific molecular biology typing tools, in order to unravel the specific Leptospira specie and serovar associated with this clinical case. A significant finding of this study was that the obtained isolate was confirmed by PCR as L. interrogans, its VNTR profile properly matching with L. interrogans Hardjoprajitno as well as its specific genomic identity revealed by secY gen.Conclusion: Leptospira interrogans serovar Hardjoprajitno was associated with the investigated calf clinical case. This information adds to the value of serologic results commonly reported, which encourage vaccination improvements to match circulating strains. In addition, this finding represents the first case report of this serovar in Chilean cattle.

Keywords

Leptospira; Cattle; Clinical; Hardjoprajitno

Published in

BMC Veterinary Research
2015, volume: 11, article number: 66
Publisher: BIOMED CENTRAL LTD

Authors' information

Salgado, Miguel
Southern University of Chile (UACh)
Otto, Barbara
Southern University of Chile (UACh)
Moroni, Manuel
Southern University of Chile (UACh)
Sandoval, Errol
Southern University of Chile (UACh)
Reinhardt, German
Southern University of Chile (UACh)
Encina, Carolina
Southern University of Chile (UACh)
Muñoz-Zanzi, Claudia
University of Minnesota

Sustainable Development Goals

SDG3 Good health and wellbeing

UKÄ Subject classification

Medical Bioscience

Publication Identifiers

DOI: https://doi.org/10.1186/s12917-015-0369-x

URI (permanent link to this page)

https://res.slu.se/id/publ/69406