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Research article2015Peer reviewed

Chemical and ultrastructural changes of ash wood thermally modified using the thermo-vacuum process: I. Histo/cytochemical studies on changes in the structure and lignin chemistry

Kim, Jong Sik; Gao, Jie; Terziev, Nasko; Cuccui, Ignazia; Daniel, Geoffrey

Abstract

Changes in structure and lignin chemistry were investigated in ash wood thermally modified (TMW) by the thermo-vacuum (Termovuoto) process for 3 h at 190-220 degrees C by means of light, fluorescence, and transmission electron (TEM) microscopy combined with histo/cytochemistry. Variation in changes in native cell color in TMWs was positively correlated with differences in lignin content between cell types and cell wall regions in the reference wood. Histochemical staining showed increasing amounts of acidic groups in TMWs with different response to ethanol extraction between secondary cell walls and CMLcc (compound middle lamella/middle lamella cell corner) regions. Fluorescence microscopy of TMWs and references showed a difference in intensity and color emission of lignin autofluorescence, reflecting modification of lignin in TMWs. Changes in histochemistry and fluorescence were prominent at and above 200 degrees C. With TEM, increased intensity of lignin staining and distortion of fiber S-1 layers were detected in TMW treated for 3 h at 220 degrees C (TMW3 h, 220 degrees C). TMW3 h, 220 degrees C differed significantly in molecular ultrastructure of fiber cell walls compared to references, such as loss of the lamellar structure and size and distribution of lignin aggregates. The modification in CMLcc structure in ash TMW3 h, 220 degrees C is different from that of softwoods.

Keywords

ash; fluorescence microscopy; lignin autofluorescence; lignin staining; syringyl/guaiacyl ratio (S/G ratio); thermally modified wood (TMW); thermo-vacuum (Termovuoto) process; transmission electron microscopy (TEM); ultrastructure

Published in

Holzforschung
2015, Volume: 69, number: 5, pages: 603-613

      SLU Authors

    • Kim, Jongsik

      • Department of Forest Products, Swedish University of Agricultural Sciences

      • Gao, Jie

        • Department of Forest Products, Swedish University of Agricultural Sciences

        • Terziev, Nasko

          • Department of Forest Products, Swedish University of Agricultural Sciences

          • Daniel, Geoffrey

            • Department of Forest Products, Swedish University of Agricultural Sciences

          UKÄ Subject classification

          Wood Science

          Publication identifier

          DOI: https://doi.org/10.1515/hf-2014-0148

          Permanent link to this page (URI)

          https://res.slu.se/id/publ/69737