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Research article2015Peer reviewed

Chemical and ultrastructural changes of ash Wood thermally modified (TMW) using the thermovacuum process: II. Immunocytochemical study of the distribution of noncellulosic polysaccharides

Kim, Jongsik; Gao, Jie; Allegretti, Ottaviano; Daniel, Geoffrey; Kim, Jong Sik

Abstract

Following structural and cytochemical studies (Part I) on thermally modified ash wood (TMW) by the thermo-vacuum (Termovuoto) process, changes in the distribution of noncellulosic polysaccharides have been investigated in TMW treated for 3 h at 220 by means of immunogold localization methods. Pectins (homogalacturonan, rhamnogalacturonan-I) and xyloglucan were significantly degraded in compound middle lamella (CML), including the middle lamella cell corner regions (CML Xylan and mannan degradation were also visible in fiber cell walls. In particular, degradation of mannan was very significant and showed variation between cell wall regions even within the same cell wall. The degradation of pectins was more significant than that of hemicelluloses. In summary, results suggest that each noncellulosic polysaccharide may have a different degradation process in ash TMWs°C (TMW3 h, 220°C)cc), of all xylem cells after thermal modification.

Keywords

ash, hemicelluloses, immunolocalization, pectins, thermally modified wood (TMW), thermo-vacuum (Termovuoto) process

Published in

Holzforschung
2015, Volume: 69, number: 5, pages: 615-625

      SLU Authors

    • Kim, Jongsik

      • Department of Forest Products, Swedish University of Agricultural Sciences
      • Gao, Jie

        • Department of Forest Products, Swedish University of Agricultural Sciences
        • Daniel, Geoffrey

          • Department of Forest Products, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Wood Science

        Publication identifier

        DOI: https://doi.org/10.1515/hf-2014-0149

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/69739