Lopez, José
- Department of Ecology, Swedish University of Agricultural Sciences
- University of Oviedo
Abstract
Noninvasive samples for genetic analyses have become essential to address ecological questions. Popular noninvasive samples such as faeces contain degraded DNA which may compromise genotyping success. Saliva is an excellent alternative DNA source but scarcity of suitable collection methods makes its use anecdotal in field ecological studies. We develop a noninvasive method of collection that combines baits and porous materials able to capture saliva. We report its potential in optimal conditions, using confined dogs and collecting saliva early after deposition. DNA concentration in saliva extracts was generally high (mean 14 ng mu l(-1)). We correctly identified individuals in 78% of samples conservatively using ten microsatellite loci, and 90% of samples using only eight loci. Consensus genotypes closely matched reference genotypes obtained from hair DNA (99% of identification successes and 91% of failures). Mean genotyping effort needed for identification using ten loci was 2.2 replicates. Genotyping errors occurred at a very low frequency (allelic dropout: 2.3%; false alleles: 1.5%). Individual identification success increased with duration of substrate handling inside dog's mouth and the volume of saliva collected. Low identification success was associated with baits rich in DNA-oxidant polyphenols and DNA concentrations
Published in
PLoS ONE
2015, volume: 10, number: 10, article number: e0139765
Publisher: Public Library of Science
SLU Authors
UKÄ Subject classification
Genetics and Genomics
Ecology
Publication identifier
- DOI: https://doi.org/10.1371/journal.pone.0139765
Permanent link to this page (URI)
https://res.slu.se/id/publ/70179