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Research article - Peer-reviewed, 2016

Microsatellite-Aided Screening for Fertility Restorer Genes (Rf) Facilitates Hybrid Improvement

El-Namaky, Raafat; Sedeek, Saber; Dea Moukoumbi, Yonnelle; Ortiz Rios, Rodomiro Octavio; Manneh, Baboucarr;


DNA markers enabled to determine the chromosomal locations of the two Rf genes (Rf3 and Rf4) in the wild abortive-cytoplasmic male sterility (WA-CMS) system. Four simple sequence repeats (SSR or microsatellites) RM171, RM258, RM315 and RM443 were used to detect the allelic status with respect to the fertility restoring genes (Rf3 and Rf4) in 300 rice cultivars and breeding lines. Furthermore, 45 lines selected using SSR markers were mated with a CMS line (IR58025A) to analyze their restoring ability. The results revealed that 90 lines had Rf3, 65 lines had Rf4 and 45 lines had Rf3 and Rf4 alleles out of 300 lines screened. Offspring of all test lines except HHZ 8-SAL9DT1-Y1, HHZ 5-SAL9-Y3-1 and IDSA 77 exhibited high pollen and spikelet fertility (> 80%), thus confirming they bear Rf alleles. the hybrid offspring of ARH12-6-1-1-B-3-1, IR32307-10-3-2-1 and Sahel329 had the highest pollen and spikelet fertility, (95.1–97, 4%, and 97.1–98.3%, 97.1-96.1% respectively).

Published in

Rice Science

2016, volume: 23, number: 3, pages: 160-164

Authors' information

El-Namaky, Raafat
Africa Rice Center (AfricaRice)
Sedeek, Saber
Africa Rice Center (AfricaRice)
Dea Moukoumbi, Yonnelle
Africa Rice Center (AfricaRice)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Manneh, Baboucarr
Africa Rice Center (AfricaRice)

UKÄ Subject classification

Genetics and Breeding
Plant Biotechnology

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