Organelles maintain spindle position in plant meiosis

Abstract

Accurate positioning of spindles is a critical aspect of cell division as it ensures that each daughter cell contains a single nucleus. In many flowering plants, two meiotic chromosome separations occur without intervening cytokinesis, resulting in two spindles in one cell during the second division. Here we report a detailed examination of two mutants, jason (jas) and parallel spindle1 (ps1), in which disturbed spindle position during male meiosis II results in the incorporation of previously separated chromosome groups into a single cell. Our study reveals that an organelle band provides a physical barrier between the two spindles. The loss of a single protein, JAS, from this organelle band leads to its disruption and a random movement of the spindles. JAS is largely associated with vesicles in the organelle band, revealing a role for vesicles in plant meiosis and that cytoplasmic events maintain spindle position during the chromosome division.

Published in

Nature Communications
2015, volume: 6, article number: 6492
Publisher: NATURE PUBLISHING GROUP

SLU Authors

• Yi, Jun

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Jiang, Hua

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Minina, Alyona

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Köhler, Claudia

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics and Genomics
Cell Biology

Publication identifier

• DOI: https://doi.org/10.1038/ncomms7492

Permanent link to this page (URI)

https://res.slu.se/id/publ/72362