Abstract

Soft rot decay of treated wood in ground contact and loss of service-life remains an important economic issue worldwide. This study determined the identity of unclassi (204-1, 35-1, TS4M3, 25M3) isolated from wood in-service. Phylogenetic analysis clustered strains into a single clade homologous with internal transcribed spacer (ITS) rDNA showed minor differences at single bases for strains 35-1 and 25M3. Compatibility occurred between TS4M3, 25M3 and 204-1 but not 35-1, lagerbergii with strain 35-1 least aggressive. Light microscopy showed Type I diffuse soft rot cavity formation reminiscent of TS4M3, 25M3 and 204-1 but always less for 35-1. Strains 35-1 and TS4M3 showed highest Cutolerance on CuSO indicate the homology and decay morphology, although considerable intra-variation exists.fied Phialophora strainsPhialocephala dimorphospora. Sequencing ofPhialophoraor Phialophora botulispora. Birch was more rapidly degraded than pine for all strains,P. dimorphospora. Growth rates at 15, 20 and 26°C were similar for4 and micronized copper incorporated into malt extract agar plates. Results indicate the Phialophora spp. A strains represent P. dimorphospora as suggested by strong strong homology and decay morphology, although considerable intra-variation exists.

Keywords

Phialophora spp. A; Soft rot; Phylogeny; PCR; Copper tolerance; ASTM soil block test; Decay testing; Phialocephala dimorphospora

Published in

International Wood Products Journal
2015, Volume: 6, number: 4, pages: 189-197

SLU Authors

• Karunasekera, Hasanthi

  • Department of Forest Products, Swedish University of Agricultural Sciences
    

• Daniel, Geoffrey

  • Department of Forest Products, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Wood Science
Other Biological Topics
Forest Science


Publication identifier

DOI: https://doi.org/10.1080/20426445.2015.1104077

Permanent link to this page (URI)

https://res.slu.se/id/publ/72725