Abstract

The establishment and maintenance of controlled auxin gradients within plant tissues are essential for a multitude of developmental processes. Auxin gradient formation is co-ordinated via local biosynthesis and transport. Cell to cell auxin transport is facilitated and precisely regulated by complex endomembrane trafficking mechanisms that target auxin carrier proteins to their final destinations. In turn, auxin and cross-talk with other phytohormones regulate the endomembrane trafficking of auxin carriers. Dissecting such rapid and complicated processes is challenging for classical genetic experiments due to trafficking pathway diversity, gene functional redundancy, and lethality in lossof-function mutants. Many of these difficulties can be bypassed via the use of small molecules to modify or disrupt the function or localization of proteins. Here, we will review examples of the knowledge acquired by the use of such chemical tools in this field, outlining the advantages afforded by chemical biology approaches.

Keywords

Auxin carriers; auxin gradients; auxin transport; chemical biology; endomembrane trafficking; phytchormones

Published in

Journal of Experimental Botany
2015, Volume: 66, number: 16, pages: 4971-4982
Publisher: OXFORD UNIV PRESS

SLU Authors

• Doyle, Siamsa

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Vain, Thomas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Robert, Stephanie

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany
Cell Biology
Developmental Biology


Publication identifier

DOI: https://doi.org/10.1093/jxb/erv179

Permanent link to this page (URI)

https://res.slu.se/id/publ/73918