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Research article - Peer-reviewed, 2005

Separation of dietary folates by gradient reversed-phase HPLC

Johansson Madelene, Jastrebova Jelena, Grahn Anders, Jägerstad Margaretha

Abstract

A study of ten silica-based stationary phases and gradient elution conditions to separate dietary folates by reversed-phase HPLC was performed. Alkyl-bonded stationary phases (both conventional and alternative) were found to be the most promising for the separation of different folate monoglutamates in terms of selectivity and peak shape. These phases were better than phenyl-bonded phases which lacked selectivity when separating 10-formyl-folic acid and 5-formyl-tetrahydrofolate. Polar-bonded (cyano) stationary phase showed similar retention characteristics as the conventional alkyl-bonded phases, but ranked below those in terms of peak shape. Overall, alternative stationary phases exhibited slightly higher retention of late-eluted folates and greater retention variability for early-eluting tetrahydrofolate and 5-methyl-tetrahydrofolate. Best selectivity was achieved on alternative polar endcapped Aquasil C-18 followed by conventional Synergy MAX C-12 and Genesis C-18 stationary phases

Keywords

column liquid chromatography; silica-based stationary phases; gradient elution conditions; folates; food analysis

Published in

Chromatographia
2005, volume: 62, number: 1-2, pages: 33-40
Publisher: Vieweg, Germany

Authors' information

Johansson, Madelene
Swedish University of Agricultural Sciences, Department of Food Science
Jastrebova, Jelena
Swedish University of Agricultural Sciences, Department of Food Science
Jägerstad, Margaretha
Swedish University of Agricultural Sciences, Department of Food Science
Grahn, Anders

UKÄ Subject classification

Food Science

URI (permanent link to this page)

https://res.slu.se/id/publ/7546