Sandquist, David
- University of Hamburg
Research article2015Peer reviewedOpen access
Anatomical and Immunocoverage Observations on SuSy, C4H, and Pectate Lyase Family Protein Down-regulated Aspens Genotypes
Sandquist, David; Norell, Lennart; Takabe, Keiji; Yoshinaga, Arata; Daniel, Geoffrey
Abstract
This study aims to complement previous studies on down-regulation of sucrose synthase and cinnamate 4-hydroxylase in hybrid aspen (Bjurhager et al. 2010; Gerber et al. 2014) with a quantitative anatomical comparison (Sandquist et al. 2015). The main focus was placed on evaluating quantitative modifications to fiber morphology and lignin composition. This was achieved by combining results from light-, electron-, and UV-microscopy together with histochemical staining. Overall there was good agreement between the morphological results in this study compared to the previously published chemical and physical reports, particularly on cinnamate 4-hydroxylase down-regulated aspen. The previously reported statistical correlations between fiber lumen area and plant diameter, and fiber length and tree height (Sandquist et al. 2015) have been further refined. Knock-down of genes coding for biologically important proteins such as sucrose synthase and cinnamate 4-hydroxylase had broad spanning morphological impacts. Down-regulated sucrose synthase genotype showed significantly reduced fiber development, possibly with altered S/G ratio. Down-regulated cinnamate 4-hydroxylase genotype showed an overall reduction of lignin in fibers as indicated from both chemical staining and UV-microscopy measurements. Down-regulated pectate lyase genotype showed no overall significant effects on fiber morphology, ultrastructural, nor lignin staining.
Keywords
Transgenic poplar, wood ultrastructure, cinnamate 4-hydroxylase (KR139), sucrose synthase (KR112), pectate lyase family protein (KR213), UV-microscopy, lignin staining, quantitative wood anatomy
Published in
Bioresources
2015, volume: 10, number: 3, pages: 5016-5029
Publisher: NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
SLU Authors
Norell, Lennart
- Department of Economics, Swedish University of Agricultural Sciences
- Department of Economics, Swedish University of Agricultural Sciences
Daniel, Geoffrey
- Department of Forest Products, Swedish University of Agricultural Sciences
- Department of Forest Products, Swedish University of Agricultural Sciences
UKÄ Subject classification
Wood Science
Forest Science
Publication identifier
- DOI: https://doi.org/10.15376/biores.10.3.5016-5029
Permanent link to this page (URI)
https://res.slu.se/id/publ/75570