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Research article - Peer-reviewed, 2015

Anatomical and Immunocoverage Observations on SuSy, C4H, and Pectate Lyase Family Protein Down-regulated Aspens Genotypes

Sandquist, David; Norell, Lennart; Takabe, Keiji; Yoshinaga, Arata; Daniel, Geoffrey


This study aims to complement previous studies on down-regulation of sucrose synthase and cinnamate 4-hydroxylase in hybrid aspen (Bjurhager et al. 2010; Gerber et al. 2014) with a quantitative anatomical comparison (Sandquist et al. 2015). The main focus was placed on evaluating quantitative modifications to fiber morphology and lignin composition. This was achieved by combining results from light-, electron-, and UV-microscopy together with histochemical staining. Overall there was good agreement between the morphological results in this study compared to the previously published chemical and physical reports, particularly on cinnamate 4-hydroxylase down-regulated aspen. The previously reported statistical correlations between fiber lumen area and plant diameter, and fiber length and tree height (Sandquist et al. 2015) have been further refined. Knock-down of genes coding for biologically important proteins such as sucrose synthase and cinnamate 4-hydroxylase had broad spanning morphological impacts. Down-regulated sucrose synthase genotype showed significantly reduced fiber development, possibly with altered S/G ratio. Down-regulated cinnamate 4-hydroxylase genotype showed an overall reduction of lignin in fibers as indicated from both chemical staining and UV-microscopy measurements. Down-regulated pectate lyase genotype showed no overall significant effects on fiber morphology, ultrastructural, nor lignin staining.


Transgenic poplar, wood ultrastructure, cinnamate 4-hydroxylase (KR139), sucrose synthase (KR112), pectate lyase family protein (KR213), UV-microscopy, lignin staining, quantitative wood anatomy

Published in

2015, volume: 10, number: 3, pages: 5016-5029

Authors' information

Sandquist, David
University of Hamburg
Norell, Lennart
Swedish University of Agricultural Sciences, Department of Economics
Takabe, Keiji
Kyoto University
Yoshinaga, Arata
Kyoto University
Swedish University of Agricultural Sciences, Department of Forest Products

UKÄ Subject classification

Wood Science
Forest Science

URI (permanent link to this page)