Johnsson, Lars
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Johnsson L, Dutta PC
The number of characterized phytosterol oxidation products (POPs) from both ring- and side-chain structures has increased during recent decades, resulting in difficulties in the separation of POPs on different gas chromatography (GC capillary columns. The main objective of this study was to separate a mixture of 29 purified and characterized oxidation products from sito-, campe- and stigmasterol using GC capillary columns with different polarity. For the first time in the area of POPs analysis, the separation efficiency of the combination of two capillary GC columns with different polarities was investigated. A non-polar 5% phenyl coated (DB5-MS) and a mid-polar 35% phenyl coated (DB35-MS) column was combined with a pressfit connector. The main improvement was enhanced base line separation for many of the analyzed POPs, compared with the separations achieved using the individual columns. However, three pairs of POPs co-eluted: 24-hydroxysitosterol/campesterol-5beta,6beta-epoxide, stiamasterol-5beta,6beta-epoxide/campesterol-5alpha,6alpha-epoxide and stigmasterol-5alpha,6alpha-epoxide/campestanetriol. (C) 2004 Published by Elsevier B.V
campesterol; coupled columns; column polarity; phytosterol oxidation products; sitosterol; stigmasterol; identification
Journal of Chromatography A
2005, Volume: 1064, number: 2, pages: 213-217
Publisher: ELSEVIER SCIENCE BV
Food Science
DOI: https://doi.org/10.1016/j.chroma.2004.12.067
https://res.slu.se/id/publ/7560