Johnsson, Lars
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
The number of characterized phytosterol oxidation products (POPs) from both ring- and side-chain structures has increased during recent decades, resulting in difficulties in the separation of POPs on different gas chromatography (GC capillary columns. The main objective of this study was to separate a mixture of 29 purified and characterized oxidation products from sito-, campe- and stigmasterol using GC capillary columns with different polarity. For the first time in the area of POPs analysis, the separation efficiency of the combination of two capillary GC columns with different polarities was investigated. A non-polar 5% phenyl coated (DB5-MS) and a mid-polar 35% phenyl coated (DB35-MS) column was combined with a pressfit connector. The main improvement was enhanced base line separation for many of the analyzed POPs, compared with the separations achieved using the individual columns. However, three pairs of POPs co-eluted: 24-hydroxysitosterol/campesterol-5beta,6beta-epoxide, stiamasterol-5beta,6beta-epoxide/campesterol-5alpha,6alpha-epoxide and stigmasterol-5alpha,6alpha-epoxide/campestanetriol. (C) 2004 Published by Elsevier B.V
Keywords
campesterol; coupled columns; column polarity; phytosterol oxidation products; sitosterol; stigmasterol; identification
Published in
Journal of Chromatography A
2005, volume: 1064, number: 2, pages: 213-217
Publisher: ELSEVIER SCIENCE BV
SLU Authors
Dutta, Paresh
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/j.chroma.2004.12.067
Permanent link to this page (URI)
https://res.slu.se/id/publ/7560