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Research article - Peer-reviewed, 2005

Separation of phytosterol oxidation products by combination of different polarity gas chromatography capillary columns

Johnsson L, Dutta PC

Abstract

The number of characterized phytosterol oxidation products (POPs) from both ring- and side-chain structures has increased during recent decades, resulting in difficulties in the separation of POPs on different gas chromatography (GC capillary columns. The main objective of this study was to separate a mixture of 29 purified and characterized oxidation products from sito-, campe- and stigmasterol using GC capillary columns with different polarity. For the first time in the area of POPs analysis, the separation efficiency of the combination of two capillary GC columns with different polarities was investigated. A non-polar 5% phenyl coated (DB5-MS) and a mid-polar 35% phenyl coated (DB35-MS) column was combined with a pressfit connector. The main improvement was enhanced base line separation for many of the analyzed POPs, compared with the separations achieved using the individual columns. However, three pairs of POPs co-eluted: 24-hydroxysitosterol/campesterol-5beta,6beta-epoxide, stiamasterol-5beta,6beta-epoxide/campesterol-5alpha,6alpha-epoxide and stigmasterol-5alpha,6alpha-epoxide/campestanetriol. (C) 2004 Published by Elsevier B.V

Keywords

campesterol; coupled columns; column polarity; phytosterol oxidation products; sitosterol; stigmasterol; identification

Published in

Journal of Chromatography A
2005, Volume: 1064, number: 2, pages: 213-217
Publisher: ELSEVIER SCIENCE BV

    SLU Authors

      • UKÄ Subject classification

      Food Science

      Publication Identifiers

      DOI: https://doi.org/10.1016/j.chroma.2004.12.067

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/7560