Abstract

Protein phosphorylation changes of the sarcoplasmic and myofibrillar proteins in beef longissimus muscle in response to electrical stimulation (ES) was investigated. Sarcoplasmic and myofibrillar proteins purified from muscle samples taken at 0, 3 and 10 h after ES were separated on SDS-PAGE and stained with phosphorous and protein specific stains. There was a significant effect of ES on phosphorylation of total sarcoplasmic and myofibrillar proteins (P < 0.05). However, although there an instant effect of ES on the phosphorylation level of the myofibrillar proteins, the ES effect on the sarcoplasmic proteins (P < 0.05) was first observed after 3 h. Several protein bands were analyzed by LC-MS/MS, revealing that the major glycolytic proteins, including glycogen debranching enzyme, glycogen phosphorylase and 6-phosphofructokinase probably are affected by ES together with different heat shock proteins. This work gives an insight into the regulation of the glycolytic enzymes and muscle contraction on application of electrical stimulation. (c) 2014 Elsevier Ltd. All rights reserved.

Keywords

Protein phosphorylation; Electrical stimulation; Beef

Published in

Food Chemistry
2015, Volume: 175, pages: 197-202
Publisher: ELSEVIER SCI LTD

SLU Authors

• Lundström, Kerstin

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology
Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1016/j.foodchem.2014.11.139

Permanent link to this page (URI)

https://res.slu.se/id/publ/76025