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Research article2005Peer reviewed

Verification of auxin-induced gene expression during adventitious rooting in rolB-transformed and untransformed apple Jork 9

Sedira M, Butler E, Gallagher T, Welander M

Abstract

Apple Jork 9 transformed with the rolB gene is more sensitive to IBA than an untransformed control. Study of the effects of IBA concentration and time of exposure on the Jork 9 stem discs shows the optimum time for root induction in the transformed plants was 7 h with 24.6 mu M IBA, compared to 7 h with 49.2 mu M IBA for untransformed stem discs. Expression of the auxin-induced adventitious rooting related oxygenase (ARRO-1) gene during adventitious rooting in rolB-transformed and untransformed apple Jork 9 has been studied. The higher expression of the ARRO-1 gene in the rolB-transformed material suggests that its expression may be rooting-specific rather than auxin-specific. (c) 2004 Elsevier Ireland Ltd. All rights reserved

Published in

Plant Science
2005, Volume: 168, number: 5, pages: 1193-1198 Publisher: ELSEVIER SCI IRELAND LTD

      SLU Authors

    • Welander, Margareta

      • Department of Crop Science, Swedish University of Agricultural Sciences

    UKÄ Subject classification

    Horticulture

    Publication identifier

    DOI: https://doi.org/10.1016/j.plantsci.2004.12.017

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/7626