Frozen-Thawed Epididymal Sperm Quality and the Success Rate of Artificial Insemination in Spotted Buffaloes (Bubalus bubalis carabanensis)

Abstract

Spotted buffalo is an exotic species in Indonesia, especially in Torajaland, South Sulawesi. Population of this species is close to become extinct due to a high slaughtering rate of this animal in the funeral ceremonies. The extinction risks are become worse since the farmer kept the bull with special treatment and prevent them from mating activity. Therefore, we tried to conserve gamete by collecting the sperm from caudal epididymides of bulls soon after slaughtering. Soya bean-based (A) and two egg yolk-based (B and C) extenders were used in order to find out the optimal freezing extender that could maintain the quality and fertility of post-thawed epididymal sperm. The post-thawed progressive motility of epididymal sperm was 45, 40 and 39.2%, while livability was 64.8, 66 and 63.3% and functional membrane integrity was 65.8, 65.4 and 63% for A, B and C extenders, respectively. In general, there were no significantly different (P>0.05) of all observed parameters in different phospholipid-based extenders. The pregnancy rate obtained from soya bean-based extender (37.5%) was also similar (P>0.05) to that of egg yolk-based (40%) extender. In conclusion, soya bean-based and egg yolk-based extenders could maintain the post-thawed quality of spotted buffalo epididymal sperm and yielded similar pregnancy rate following artificial insemination program.

Keywords

Epididymal Sperm; Artificial Insemination; Spotted Buffalo

Published in

Buffalo Bulletin
2013, volume: 32, number: Special Issue 2, pages: 494-497
Publisher: INT BUFFALO INFORMATION CTR

SLU Authors

• Yusniza, yulnawati

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • Bogor Agricultural University
  • Indonesian Institute of Sciences (LIPI)

UKÄ Subject classification

Animal and Dairy Science

Permanent link to this page (URI)

https://res.slu.se/id/publ/76876