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Research article - Peer-reviewed, 2016

Quantitative PCR shows propagation of Plasmodiophora brassicae in Swedish long term field trials

Jonsson, Anders; Marzec-Schmidt, Katarzyna; Börjesson, Gunnar; Wallenhammar, Ann-Charlotte

Abstract

Clubroot (Plasmodiophora brassicae) is a serious soil-borne disease in brassica crops world-wide. We report on a time series of soil samples from Swedish long-term fertility trials started in 1957, 1963 and 1966, which were analyzed for the amount ofP. brassicaeDNA. The crop rotations included a brassica crop every 4 or 6years. All experimental sites with a 4-year rotation of oilseed rape, except one with calcium carbonate in the soil profile, showed high (>1000fg DNA g−1soil) levels ofP. brassicaeDNA after 9, 11 and 12 rotations. In contrast, detectable levels (>5fg DNA g−1soil) ofP. brassicaewere found only at one of five sites with a 6-year rotation of spring oilseed rape. In years with high levels ofP. brassicaeDNA, low yield was reported and a subsequent decline inP. brassicaeDNA in soil was observed. Different NPK (nitrogen/phosphorus/potassium) fertiliser regimes resulted in similarP. brassicaeDNA levels. The robustness and reliability of the method applied was verified by analyses of soil from individual plots compared with a mixture of plots and by repeated analyses of selected samples, which showed thatP. brassicaeDNA remained stable during dry storage.

Keywords

Long-termfertility; field trials; Clubroot; Soil samples; Plasmodiophora brassicae; Quantitative PCR; qPCR

Published in

European Journal of Plant Pathology
2016, volume: 145, number: 3, pages: 573-581
Publisher: Springer Verlag (Germany)

Authors' information

Swedish University of Agricultural Sciences, Department of Soil and Environment
Swedish University of Agricultural Sciences, Department of Soil and Environment
Swedish University of Agricultural Sciences, Department of Soil and Environment
Swedish University of Agricultural Sciences, Department of Soil and Environment

Associated SLU-program

SLU Network Plant Protection

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1007/s10658-016-0906-x

URI (permanent link to this page)

https://res.slu.se/id/publ/77245