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Research article2016Peer reviewed

Induced defenses change the chemical composition of pine seedlings and influence meal properties of the pine weevil Hylobius abietis

Lundborg, Lina; Fedderwitz, Frauke; Björklund, Niklas; Nordlander, Göran; Borg-Karlson, Anna-Karin

Abstract

The defense of conifers against phytophagous insects relies to a large extent on induced chemical defenses. However, it is not clear how induced changes in chemical composition influence the meal properties of phytophagous insects (and thus damage rates). The defense can be induced experimentally with methyl jasmonate (MeJA), which is a substance that is produced naturally when a plant is attacked. Here we used MeJA to investigate how the volatile contents of Scots pine (Pinus sylvestris L.) tissues influence the meal properties of the pine weevil (Hylobius abietis (L.)). Phloem and needles (both weevil target tissues) from MeJA-treated and control seedlings were extracted by n-hexane and analyzed by two-dimensional gas chromatography-mass spectrometry (2D GC-MS). The feeding of pine weevils on MeJA-treated and control seedlings were video-recorded to determine meal properties. Multivariate statistical analyses showed that phloem and needle contents of MeJA-treated seedlings had different volatile compositions compared to control seedlings. Levels of the pine weevil attractant (+)-alpha-pinene were particularly high in phloem of control seedlings with feeding damage. The antifeedant substance 2-phenylethanol occurred at higher levels in the phloem of MeJA-treated than in control seedlings. Accordingly, pine weevils fed slower and had shorter meals on MeJA-seedlings. The chemical compositions of phloem and needle tissues were clearly different in control seedlings but not in the MeJA-treated seedlings. Consequently, meal durations of mixed meals, i.e. both needles and phloem, were longer than phloem meals on control seedlings, while meal durations on MeJA seedlings did not differ between these meal contents. The meal duration influences the risk of girdling and plant death. Thus our results suggest a mechanism by which MeJA treatment may protect conifer seedlings against pine weevils. (C) 2016 Elsevier Ltd. All rights reserved.

Keywords

Pinus sylvestris; Pinaceae; Hylobius abietis; Short-term feeding; Induced defense; Methyl jasmonate; Phloem; Needles; Terpenes; Aromatics

Published in

Phytochemistry
2016, Volume: 130, pages: 99-105 Publisher: PERGAMON-ELSEVIER SCIENCE LTD

      SLU Authors

      • Associated SLU-program

        SLU Plant Protection Network

        UKÄ Subject classification

        Organic Chemistry
        Ecology

        Publication identifier

        DOI: https://doi.org/10.1016/j.phytochem.2016.06.002

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/77545