Effect of sperm preparation on development of bovine blastocyst in vitro

Abstract

colloids has been used to select normal sperm for assisted reproduction in several species. Animal models can sometimes be used as a preliminary step to investigate sperm preparation methods that are potentially of use for human fertility treatments. In this study bovine semen was prepared using three variants of the single-layer centrifugation sperm selection technique (Small, Mini, Mini-EP) with Bovicoll (Androcoll-B). Computer-assisted sperm motility analysis, the hypo-osmotic swelling test, and the sperm chromatin structure assay were performed on unselected (control) and SLC-selected sperm samples. Mini and Mini-EP gave the highest yield of motile spermatozoa, progressive motility and membrane integrity. In vitro fertilization trials were performed to investigate the fertilizing ability of the frozen-thawed bovine spermatozoa selected with Bovicoll. Mini-SLC (single-layer centrifugation) and swim-up (Control) were performed and cleavage rate and blastocyst rate did not differ significantly between groups. As there was a trend to an increased number of cells in blastocysts in the SLC group, the Mini-SLC method is at least as good as swim-up for selecting frozen-thawed bull spermatozoa for in vitro fertilization (IVF). This method could potentially be used to prepare human sperm for assisted reproduction.

Keywords

Bovine; Computer-assisted semen analysis (CASA); In vitro fertilization (IVF); In vitro production (IVP) of embryos; Single-layer centrifugation; Sperm chromatin structure assay (SCSA)

Published in

Zygote
2016, volume: 24, number: 6, pages: 825-830

SLU Authors

• Abraham, Maria Celina

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Johannisson, Anders

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Morrell, Jane

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

Associated SLU-program

Translational and comparative medicine

UKÄ Subject classification

Genetics and Breeding in Agricultural Sciences
Other Veterinary Science

Publication identifier

• DOI: https://doi.org/10.1017/S0967199416000150

Permanent link to this page (URI)

https://res.slu.se/id/publ/77721