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Research article - Peer-reviewed, 2016

Comparison of differents methods of sperm selection of llama raw semen

Santa Cruz, R.; Giuliano, S; Gambarotta, M. C.; Morrell, Jane; Abraham, Maria Celina; Miragaya, M; Carretero, M


The objective of this study was to compare the efficiency of different sperm selection methods applied to the same llama ejaculate. Four treatments were compared: two variants of the swim up technique (with and without seminal plasma), and two different colloids, Androcoll-E-Large and Percoll (R). Using electroejaculation, 21 semen samples were obtained from 7 llama males (n = 7, r=3). The ejaculates were incubated in a solution of 0.1% collagenase, to decrease thread formation, and then split into 4 aliquots: one aliquot was layered over a column of Androcoll-E-Large (SLC) and the second over a column of Percoll (45%). The third aliquot was deposited in a tube with culture medium and was incubated at a 45 degrees angle for 30 min at 37 degrees C (SU1). The last aliquot was centrifuged to separate the spermatozoa and seminal plasma. The sperm pellet obtained was resuspended, and transferred to a tube with culture medium which was incubated at an angle of 45 degrees for 30 min at 37 degrees C (SU2). Both aliquots SLC and P showed higher proportions of progressive motility and plasma membrane functionality (p <= 0.05) than raw semen. There were no significant differences (p > 0.05) in sperm viability and in normal spermatozoa between raw semen and treatments. Nevertheless, only SLC did not have a significant increase of bent tails. In conclusion SLC centrifugation would be the method of choice for selecting llama spermatozoa.


Sperm selection; Androcoll; Percoll; Swim up; Llama

Published in

Animal Reproduction Science
2016, Volume: 173, pages: 8-12

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      Clinical Science

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