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Research article - Peer-reviewed, 2008

Regeneration from leaf segments of in vitro-grown shoots of Malus baccata

Sun, Chun Yu; Wang, Yi; Xu, Xue Feng; Sun, Yang; Zhu, Li Hua; Han, Zhen Hai

Abstract

Adventitious shoot regeneration was investigated using leaf explants of in vitro Cultured shoots of Malus baccata. Optimal regeneration was obtained using Murashige and Skoog medium supplemented with 4 mg/litre 6-benzyladenine and 0.5 mg/litre alpha-naphthalene-acetic acid. Dark incubation for 10 days gave the best results of adventitious shoot regeneration. Sorbitol was proved to be the most suitable carbon source for shoot regeneration in M. baccata. Gelling with either Phytagel or Gelrite was more efficient than agar in shoot regeneration. T highest regeneration frequency was 97% and the greatest shoot number per regenerated lea explant was 8.7. The addition of antibiotics Kanamycin or Hygromycin significantly reduced regeneration capacity in most instances. The development of the regeneration protocol in this study will facilitate the future transformation work in this species.

Keywords

Malus baccata; plant growth regulator; gelling agent; sugar; antibiotics; shoot regeneration

Published in

New Zealand Journal of Crop and Horticultural Science
2008, Volume: 36, number: 4, pages: 233-238
Publisher: RSNZ PUBLISHING

      SLU Authors

    • Zhu, Li-Hua

      • Department of Crop Science, Swedish University of Agricultural Sciences

    UKÄ Subject classification

    Horticulture

    Publication identifier

    DOI: https://doi.org/10.1080/01140670809510239

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/77815