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Research article - Peer-reviewed, 2016

Enzyme immobilization on a nanoadsorbent for improved stability against heavy metal poisoning

Pogorilyi, Roman; Melnyk, Inna; Zub, Yuriy; Seisenbaeva, Gulaim; Kessler, Vadim

Abstract

Magnetic nanoparticles modified with siloxane layers bearing amino and thiol functions have been used for immobilization of urease either by adsorption or via surface grafting. The activity of the immobilized enzyme in the hydrolysis of urea extended to the levels typical of the native enzyme, while its long-term stability in combination with magnetic retraction opened for its repeated use in both analysis and detoxification of bio-fluids. The immobilized urease revealed strongly enhanced stability and 65% activity in the presence of 0.1 mmol/l of Hg2+ or 0.3 mmol/l of Cu2+ while the native urease did not retain any activity at all. The enzyme grafting was shown to be a potentially perspective tool in alleviation of heavy metal poisoning and to be providing an opportunity for use of the developed adsorbents as both biosensors and bio-reactants for removal of urea from biofluids. (C) 2016 Elsevier B.V. All rights reserved.

Keywords

Magnetic nano adsorbent; Urease; Enzyme activity; Urea; Heavy metal; Surface modification

Published in

Colloids and Surfaces B: Biointerfaces
2016, Volume: 144, pages: 135-142
Publisher: ELSEVIER SCIENCE BV

        SLU Authors

      • Seisenbaeva, Gulaim

        • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
        • Kessler, Vadim

          • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Physical Chemistry
        Medical Materials
        Materials Chemistry

        Publication identifier

        DOI: https://doi.org/10.1016/j.colsurfb.2016.04.003

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/78464