Research article - Peer-reviewed, 2000
A novel IS-like element frequently inserted in a putative virulence regulator in bovine mastitis isolates of Streptococcus dysgalactiae
Vasi, József; Lindberg, Martin; Guss, BengtAbstract
Streptococcus dysgalactiae, a Lancefield group C streptococcus, is commonly isolated from bovine mastitis. We recently identified a putative regulon in two S. dysgalactiae strains, 8215 and Epi9, consisting of two consecutive genes, dmg and dem, coding for a possible regulatory protein and an M-like protein with fibrinogen- and IgG-binding-properties, respectively. During these studies a short sequence homologous to an IS element was found to be inserted in the dmg gene of strain 8215. The present investigation describes the complete sequence of this IS-like clement, named ISSdyl, which consists of 1218 bp and contains two ORFs, flanked by imperfect repents. The nucleotide sequence of the IS-like element shows 82% identity to the previously reported sequence of IS/99 from Streptoccus mutans V403. The deduced amino acid sequences of the ORFs also revealed high homology to transposases from IS elements in Enterococcus faccium, Escherichia coli, and Shigella dysenteriae, all belonging to the IS3 family. We studied the distribution of ISSdyl in 57 S. dysgalactiae isolates using PCR analysis with specific primers derived from the IS element. Ninety-eight percent of the isolates contained the ISSdyl element. Surprisingly, in the majority of studied strains a copy of the IS-like element was found to be inserted in the dmg gene, a putative virulence regulator. (C) 2000 Academic Press.Keywords
Streptococcus dysgalactiae; fibrinogen binding; IgG binding; M-like protein; gene cloning; IS elementPublished in
Plasmid2000, volume: 44, number: 3, pages: 220-230
Publisher: ACADEMIC PRESS INC
Authors' information
Vasi, József
Swedish University of Agricultural Sciences, Department of Microbiology
Lindberg, Martin
SLU - Swedish University of Agricultural Sciences
Swedish University of Agricultural Sciences, Department of Microbiology
UKÄ Subject classification
Microbiology
Biochemistry and Molecular Biology
Medical Bioscience
Publication Identifiers
DOI: https://doi.org/10.1006/plas.2000.1481
URI (permanent link to this page)
https://res.slu.se/id/publ/79297