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Research article - Peer-reviewed, 1999

Pulsed-field gel electrophoresis and distribution of the genes zag and fnz in isolates of Streptococcus equi

Lindmark, H; Jonsson, P; Engvall, EO; Guss, B


Streptococcus equi subsp. equi and subsp, zooepidemicus are important pathogens of the equine respiratory tract. Isolates of both subspecies were examined by pulsed-field gel electrophoresis (PFGE). With the exception of eight isolates, a unique band pattern was displayed for each of the 48 subsp. zooepidemicus isolates tested. A method to distinguish isolates of the genetically very homogeneous subsp. equi has hitherto not been available, although several methods have been tested. By the use of PFGE, 50 isolates of subsp. equi could be divided into eleven groups, each with a unique pulsotype. In addition, the recently characterised genes encoding the cell-wall proteins ZAG and FNZ of S. equi subsp. zooepidemicus strain ZV were shown by Southern blots to be present in all 98 tested isolates, including the type strains of the two subspecies. Binding assays showed that the expression of the two genes clearly differentiate between the two subspecies.

Published in

Research in Veterinary Science
1999, volume: 66, number: 2, pages: 93-99

Authors' information

Lindmark, Hans
Swedish University of Agricultural Sciences, Department of Microbiology
Jonsson, P.
SLU - Swedish University of Agricultural Sciences
Olsson, Engvall Eva (Engvall Olsson, Eva)
National Veterinary Institute (SVA)
Swedish University of Agricultural Sciences, Department of Microbiology

UKÄ Subject classification

Biochemistry and Molecular Biology

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