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Research article - Peer-reviewed, 2016

TRPA5, an Ankyrin Subfamily Insect TRP Channel, is Expressed in Antennae of Cydia pomonella (Lepidoptera: Tortricidae) in Multiple Splice Variants

Cattaneo, Alberto M.; Bengtsson, Jonas M.; Montagne, Nicolas; Jacquin-Joly, Emmanuelle; Rota-Stabelli, Omar; Salvagnin, Umberto; Bassoli, Angela; Witzgall, Peter; Anfora, Gianfranco;

Abstract

Transient receptor potential (TRP) channels are an ancient family of cation channels, working as metabotropic triggers, which respond to physical and chemical environmental cues. Perception of chemical signals mediate reproductive behaviors and is therefore an important target for sustainable management tactics against the codling moth Cydia pomonella L. (Lepidoptera: Tortricidae). However, olfactory behavior strongly depends on diel periodicity and correlation of chemical with physical cues, like temperature, and physical cues thus essentially contribute to the generation of behavioral response. From an antennal transcriptome generated by next generation sequencing, we characterized five candidate TRPs in the codling moth. The coding DNA sequence of one of these was extended to full length, and phylogenetic investigation revealed it to be orthologous of the TRPA5 genes, reported in several insect genomes as members of the insect TRPA group with unknown function but closely related to the thermal sensor pyrexia. Reverse transcription PCR revealed the existence of five alternate splice forms of CpTRPA5. Identification of a novel TRPA and its splice forms in codling moth antennae open for investigation of their possible sensory roles and implications in behavioral responses related to olfaction.

Keywords

transient receptor potential cation channel; TRPA subfamily; TRPA5; splice form; Cydia pomonella

Published in

Journal of Insect Science

2016, volume: 16, number: 1, article number: 83
Publisher: OXFORD UNIV PRESS INC

Authors' information

Cattaneo, Alberto M.
Edmund Mach Foundation
Bengtsson, Jonas M.
Stockholm University
Montagne, Nicolas
Pierre-and-Marie-Curie University (UPMC)
Jacquin-Joly, Emmanuelle
National Institute of Agricultural Research (INRA)
Rota-Stabelli, Omar
Edmund Mach Foundation
Salvagnin, Umberto
Edmund Mach Foundation
Bassoli, Angela
University of Milano, Italy
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Anfora, Gianfranco
Edmund Mach Foundation

UKÄ Subject classification

Behavioral Sciences Biology
Biochemistry and Molecular Biology
Ecology

Publication Identifiers

DOI: https://doi.org/10.1093/jisesa/iew072

URI (permanent link to this page)

https://res.slu.se/id/publ/79357