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Book chapter, 2014

Using a Reverse Genetics Approach to Investigate Small-Molecule Activity

Doyle, Siamsa; Robert, Stephanie


Chemical genomics is a highly effective approach for understanding complex and dynamic biologicalprocesses in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a hugeabundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here wepresent an approach to characterize a chemical of interest, as well as examples of studies demonstrating aneffective combination of chemical genomics with reverse genetics strategies, drawn from recent literatureon phytohormone signalling and auxin transport.


Chemical genomics; Arabidopsis mutants; Reverse genetics; Phytohormone signalling; Auxin transport

Published in

Methods in Molecular Biology
2014, Volume: 1056, number: 1056, pages: 51-62
Book title: Plant chemical genomics : methods and protocols
ISBN: 978-1-62703-591-0, eISBN: 978-1-62703-592-7
Publisher: Springer