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Research article - Peer-reviewed, 2016

2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis

Eyer, Luděk; Robert, Stephanie; Novak, Ondrej


The herbicide 2,4-D exhibits an auxinic activity and therefore can be used as a synthetic and traceable analog to study auxin-related responses. Here we identified that not only exogenous 2,4-D but also its amide-linked metabolite 2,4-D-Glu displayed an inhibitory effect on plant growth via the TIR1/AFB auxin-mediated signaling pathway. To further investigate 2,4-D metabolite conversion, identity and activity, we have developed a novel purification procedure based on the combination of ion exchange and immuno-specific sorbents combined with a sensitive liquid chromatography-mass spectrometry method. In 2,4-D treated samples, 2,4-D-Glu and 2,4-D-Asp were detected at 100-fold lower concentrations compared to 2,4-D levels, showing that 2,4-D can be metabolized in the plant. Moreover, 2,4-D-Asp and 2,4-D-Glu were identified as reversible forms of 2,4-D homeostasis that can be converted to free 2,4-D. This work paves the way to new studies of auxin action in plant development.

Published in

2016, volume: 11, number: 7

Authors' information

Eyer, Luděk
Veterinary Research Institute
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
The Czech Academy of Sciences

UKÄ Subject classification

Biochemistry and Molecular Biology

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