Karady, Michal
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Palacký University Olomouc
Research article2016Peer reviewed
Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots
Jiskrová, Eva; Novak, Ondrej; Pospíšilová, Hana; Holubová, Katarína; Karady, Michal; Galuszka, Petr; Robert, Stephanie; Frébort, Ivo
Abstract
The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.
Published in
New Biotechnology
2016, Volume: 33, number: 5, pages: 735-742
Publisher: ELSEVIER SCIENCE BV
Robert, Stephanie
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
SLU Authors
UKÄ Subject classification
Biochemistry and Molecular Biology
Publication identifier
DOI: https://doi.org/10.1016/j.nbt.2015.12.010
Permanent link to this page (URI)
https://res.slu.se/id/publ/79534