Review article - Peer-reviewed, 2016
Next-generation sequencing workflows in veterinary infection biology: towards validation and quality assurance
Van Borm, S.; Wang, J; Granberg, Fredrik; Colling, A.Abstract
Recent advancements in DNA sequencing methodologies and sequence data analysis have revolutionised research in many areas of biology and medicine, including veterinary infection biology. New technology is poised to bridge the gap between the research and diagnostic laboratory. This paper defines the potential diagnostic value and purposes of next-generation sequencing (NGS) applications in veterinary infection biology and explores their compatibility with the existing validation principles and methods of the World Organisation for Animal Health. Critical parameters for validation and quality control (quality metrics) are suggested, with reference to established validation and quality assurance guidelines for NGS-based methods of diagnosing human heritable diseases. Although most currently described NGS applications in veterinary infection biology are not primary diagnostic tests that directly result in control measures, this critical reflection on the advantages and remaining challenges of NGS technology should stimulate discussion on its diagnostic value and on the potential to validate NGS methods and monitor their diagnostic performance.Keywords
Molecular diagnostics; Next-generation sequencing; Quality assurance; ValidationPublished in
Revue Scientifique et Technique- Office International des Epizooties2016, volume: 35, number: 1, pages: 67-81
Publisher: OFFICE INT EPIZOOTIES
Authors' information
Van Borm, S.
Wang, J
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Colling, A.
Commonwealth Scientific and Industrial Research Organisation (CSIRO)
UKÄ Subject classification
Microbiology
Pathobiology
Diagnostic Biotechnology
Publication Identifiers
DOI: https://doi.org/10.20506/rst.35.1.2418
URI (permanent link to this page)
https://res.slu.se/id/publ/79568