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Research article - Peer-reviewed, 2016

Genetic identification of a non-native species introgression into wild population of the field cricket Gryllus campestris (Orthoptera: Gryllidae) in Central Europe

Panagiotopoulou, Hanna; Baca, Mateusz; Baca, Katarzyna; Sienkiewicz, Pawel; Slipinski, Piotr; Zmihorski, Michal


Two species of the genus Gryllus occur in Europe: G. campestris and G. bimaculatus. The first is widely distributed in the north-western Palaearctic, while the second, G. bimaculatus, occurs predominantly in the Mediterranean area. There is a visible pattern in the distribution of G. campestris, the insect being rare and threatened in the western part of its range, whereas it is still abundant in the east. Despite the fact that this species is commonly used in laboratory experiments, its natural populations are poorly characterised. In the present study, we analysed cricket populations from the lower Oder and Vistula River valleys in Poland. Based on the phylogeny of the mtDNA cytochrome b fragment, we found that 17% of the individuals studied had a G. bimaculatus-like mtDNA haplotype. Analyses of 11 autosomal microsatellite loci failed to reveal any clear genetic differentiation between individuals assigned to these two clades. This suggests, along with the spatial distribution of G. bimaculatus-like haplotypes, successful interbreeding of G. bimaculatus with native populations of G. campestris. However, both the nuclear data and additional analyses of two X-chromosome-linked microsatellite loci revealed incomplete introgression. Human-mediated introgression seems to be the most plausible explanation of the observed genotypic pattern such that caution needs to be taken in conservation efforts carried out in the western part of the species' range.


Orthoptera; Gryllidae; Gryllus campestris; Gryllus bimaculatus; conservation; mtDNA; microsatellite loci; X-chromosome-linked markers

Published in

European Journal of Entomology
2016, volume: 113, pages: 446-455

Authors' information

Panagiotopoulou, H.
Polish Academy of Sciences (PAN)
Baca, Mateusz
University of Warsaw
Baca, Katarzyna
University of Warsaw
Sienkiewicz, Pawel
Poznan University of Life Sciences
Slipinski, Piotr
Polish Academy of Sciences (PAN)
Zmihorski, Michal
Polish Academy of Sciences (PAN)
Zmihorski, Michal
Swedish University of Agricultural Sciences, Department of Ecology

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