Egertsdotter, Ulrika
- Department of Forest Genetics, Swedish University of Agricultural Sciences
Abstract
Embryogenic cell-lines of Picea abies were divided into two groups based on their growth habit and the morphology of the somatic embryos. Group A grew well on solid medium and the head regions of the somatic embryos were dense, while group B grew well in suspension and the head region of the somatic embryos consisted of loosely packed cells.Protoplasts from all cell-lines could divide and enter the embryogenic pathway. The highest proportion of dividing cells entering the embryogenic pathway was obtained from cell-lines belonging to group A cultured on solid medium. However, only the protoplast derived colonies from cell-lines belonging to group B growing as suspensions continued to grow and gave rise to many somatic embryos.New embryogenic suspension cultures were established from protoplasts of one cell-line belonging to group B. Growth habit and embryo morphology were similar for the re-established suspension and the initial culture.
Keywords
SOMATIC EMBRYOGENESIS; EMBRYOGENIC CELL-LINES; NORWAY SPRUCE; PICEA-ABIES; PROTOPLAST REGENERATION
Published in
Journal of Plant Physiology
1993, volume: 141, number: 2, pages: 222-229
Publisher: GUSTAV FISCHER VERLAG
SLU Authors
Von Arnold, Sara
- Department of Forest Genetics, Swedish University of Agricultural Sciences
- Department of Forest Genetics, Swedish University of Agricultural Sciences
UKÄ Subject classification
Cell Biology
Publication identifier
- DOI: https://doi.org/10.1016/S0176-1617(11)80764-9
Permanent link to this page (URI)
https://res.slu.se/id/publ/79934