Abstract

Embryogenic cell-lines of Picea abies were divided into two groups based on their growth habit and the morphology of the somatic embryos. Group A grew well on solid medium and the head regions of the somatic embryos were dense, while group B grew well in suspension and the head region of the somatic embryos consisted of loosely packed cells.Protoplasts from all cell-lines could divide and enter the embryogenic pathway. The highest proportion of dividing cells entering the embryogenic pathway was obtained from cell-lines belonging to group A cultured on solid medium. However, only the protoplast derived colonies from cell-lines belonging to group B growing as suspensions continued to grow and gave rise to many somatic embryos.New embryogenic suspension cultures were established from protoplasts of one cell-line belonging to group B. Growth habit and embryo morphology were similar for the re-established suspension and the initial culture.

Keywords

SOMATIC EMBRYOGENESIS; EMBRYOGENIC CELL-LINES; NORWAY SPRUCE; PICEA-ABIES; PROTOPLAST REGENERATION

Published in

Journal of Plant Physiology
1993, Volume: 141, number: 2, pages: 222-229
Publisher: GUSTAV FISCHER VERLAG

SLU Authors

• Egertsdotter, Ulrika

  • Department of Forest Genetics, Swedish University of Agricultural Sciences
    

• Von Arnold, Sara

  • Department of Forest Genetics, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology


Publication identifier

DOI: https://doi.org/10.1016/S0176-1617(11)80764-9

Permanent link to this page (URI)

https://res.slu.se/id/publ/79934